王 晨^1，2，曹 菁^1，2*，郝江伟^1，2，宋娟娟^1，2，黄 盼^1，2

（1.劲牌持正堂药业有限公司，黄石 435000；

2.中药保健食品与安全湖北省重点实验室，大冶 435100）

摘要：本研究在单因素实验基础上采用正交试验优化，考察提取温度、提取时间和液料比对复方健脾养胃方中粗多糖提取率的影响，并考察纯化工艺对粗多糖保留率、浊度和感官评价的影响。结果表明，复方健脾养胃方中粗多糖的提取最优工艺为：95 ℃水浸提，液料比12∶1 mL/g，提取时间2 h，提取2次。优选的纯化工艺为：先将水提取液浓缩至相对密度为1.05～1.10（65 ℃），8 000 r/min离心20 min后用孔径为0.45 μm滤袋过滤，过滤液中粗多糖保留率为90.10%，浊度为31.4 NTU，液体澄清透明，水提液稳定性好。

关键词：复方健脾养胃方；提取工艺；纯化工艺；粗多糖

中图分类号：TS218   文献标识码：A   文章编号：1674-506X（2024）03-0049-0006

Optimization of Extraction Process and Purification Process of Compound Jianpi Yangwei Formula

WANG Chen^1，2, CAO Jing^1，2*, HAO Jiangwei^1，2, SONG Juanjuan^1，2, HUANG Pan^1，2

（1.Jing Brand Chizhengtang Pharmaceutical Co., Ltd., Huangshi 435000, China;

2.Hubei Provincial Key Laboratory for Quality and Safety of Traditional Chinese Medicine Health Food, Daye 435100, China）

Abstract：On the basis of single factor experiment, orthogonal experimental optimization was used to investigate the effects of extraction temperature, extraction time, and liquid to material ratio on the extraction rate of crude polysaccharides in the compound Jianpi Yangwei fomula, and to investigate the effects of purification process on the retention rate, turbidity, and sensory evaluation of crude polysaccharides. The results showed that the optimal extraction conditions of crude polysaccharides were as follows：water extraction at 95 ℃, the ratio of liquid to material was 12∶1 mL/g, the extraction time was 2 h, and the extraction was twice. The optimum purification process was: the water extract was concentrated to a relative density of 1.05～1.10 (65 ℃), centrifuged at 8 000 r/min for 20 min, and then filtered with a filter bag with a pore size of 0.45 μm. The retention rate of crude polysaccharide in the filtrate was 90.10%, the turbidity was 31.4 NTU, the liquid was clear and transparent, and the stability of the water extract was good.

Keywords：Jianpi Yangwei formula; extraction process; purification process; crude polysaccharides

doi：10.3969/j.issn.1674-506X.2024.03-008

收稿日期：2023-08-09

基金项目：湖北省科技重大专项课题（No.2020ACA007）

作者简介：王晨（1987-），女，硕士，工程师。研究方向：中药保健食品。

*通信作者：曹菁（1987-），女，硕士，工程师。研究方向：中药保健食品。

引用格式：王晨,曹菁,郝江伟,等.复方健脾养胃方提取工艺的优化及纯化工艺的优选[J].食品与发酵科技,2024,60(3):49-54.